Oxidation
Testing
This involves subjecting a blood sample or a suspension of red blood cells to radical aggression under strictly controlled and standardized conditions. All the enzymatic and chemical systems in the sample are mobilized to protect the integrity of the cells until they lyse.
The analysis, carried out in 96-well microplates, allows the processing of a large number of samples. Measuring the decrease in absorbance allows the progressive disappearance of cells to be monitored. The blood’s resistance to free radical attack is expressed by the time required for 50% of blood cells to lyse.
To study the overall antioxidant defense capacity of a product, a sample of the product is added to the reaction medium and the change in the resistance of the red blood cells in a control blood is then measured. The antioxidant defense capacity is given by the increase in the duration of resistance to hemolysis compared to a control. The results are standardized in Trolox equivalents (soluble equivalent of vitamin E) or gallic acid (phenolic compound).
Antioxidant defense potential of an individual
or product
Used in human or animal biology, it allows us to assess, as a preventive measure against a pathology, the shift in the individual’s defense capital towards a low pre-pathological value. The KRL™ test takes into account an individual’s genetic heritage in relation to their antioxidant defense system, their lifestyle, and known or unknown aggression situations.